In various field such as decomposition of dioxin, it’s really expected to utilize activity of microbes. But it’s difficult to monitor the microbe and to identify proper incubation conditions. So we studied on a new system to make it possible quickly and easily by using a bio-microchip. In this paper, we report about this system and the Synchronized Laser Micromanipulation(SLM) which we proposed for the trajectory control of the multiple Micro-tools by the single laser.