We examined CAMP and CGMP to evaluate the effects of acupuncture. Serum CAMP and GMP were measured, before and after stimulation, with radioimmunoassay. Both sides Tsu-San-Li were punctured for 15 minutes with 30 gauge Chinese needles, which were electrically stimulated by Chinese made GT-6805 with the setting of 1 Hz, power index 1. In normal subjects (8 males and 16 females) serum CAMP and CGMP was 20.77±2.368 pmol/ml and 13.02±1.496 pmol/ml respectively. A/G ratio was 1.609±0.217. Other normal subjects (8 males and 6 females) received acupuncture. Before stimulation CAMP was 22.95±7.607 pmol/ml, CGMP was 17.60±4.106 pmol/ml and A/G ratio was 1.326±0.432. After stimulation CAMP was 19.21±3.762 pmol/ml, CGMP was 19.92±0.454 pmol/ml and A/G ratio was 1.049±0.344. After simulation CAMP was significantly decreased, but those in low levels were increased and those in high levels were decreased. Effect of acupuncture on CGMP was not significant. After stimulation A/G ratio was decreased.