Following the digestion in vitro of soybean paste, the study in vivo was carried out on the young animal. After the 20 hours starvation, the rats about 50 days old were fed the mashed soybean for 10 minutes.
They were killed at 30, 60, 90, 120 and 180 minutes after the feeding and the smear specimens of the stomach, doudenum, ileum, large intestine and colon contents were prepared according to the method discribed in Part I.
The disappearance of stain reactive compounds from the soybean cells were more marked in the digestion in vivo than in the artificial digestion, perhaps partly due to the predigestion in the mouth and the more favorable gastric-intestinal enviroment than that in the test tube. During the digestive process in the stomach and duodenum, the soybean cells raptured, emanicipating and exposing the cell contents to direct contact with the digestive juices.