By way of the multiple layer centrifugation method which is mediated by solution of Gum Akashia and centrifuged for half an hour at 3, 000 r. p.m., cancer cells are separated from the material and the obstruction of cytodiagnosis are removed.
The distribution of the specific gravities of the solution floating cancer cells are not always simillar in all cases, but dissimillar in the kinds of the material.
In effusions, cancer cells are distributed at widest region.(10221060)
In sputum, they are distributed at narrow (1040-1055) than the mentioned above.
In materials of stomach are most limited.(1045-4055)
This dissimillarity of distribution is effected by the rate of keeping tho various degenerated cancer cells and by the general status on the experimental fluids.
The specific gravities of the solutions which are used clinically are enough with four solutions (1030, 1040, 1048, 1050) on effusions, and with three solutions (1040, 1045, 1055) on the sputa and the materials from stomach.