Abstract—
A method has been developed for the immobilization of short DNA sequences in agarose cells fixed on the surface of a polymer substrate, with their subsequent partial cleavage from agarose when heated while protecting functional properties. The effectiveness of the method was demonstrated on oligonucleotides, which, after cleavage from agarose, retained the ability to participate as primers in the PCR amplification of the sequence site 7 of the exon of the human ABO gene.
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This work was supported by the Russian Foundation for Basic Research (project no. 20-04-00680).
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Abbreviations: PET, polyethylene terephthalate; COC, cyclic olefin copolymer.
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Zolotov, A.M., Miftakhov, R.A., Ikonnikova, A.Y. et al. Biochip with Agarose Microcells Containing Thermally Separable Primers. Russ J Bioorg Chem 48, 858–863 (2022). https://doi.org/10.1134/S1068162022040203
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DOI: https://doi.org/10.1134/S1068162022040203