Issue 20, 2016
From the journal:

Lab on a Chip

Polymerase chain reaction in microfluidic devices

Christian D. Ahrberg,a   Andreas Manzbc  and  Bong Geun Chung*a  

* Corresponding authors

a Department of Mechanical Engineering, Sogang University, Seoul, Korea
E-mail: bchung@sogang.ac.kr

b Microfluidics group, KIST-Europe, Saarbrücken, Germany

c Mechanotronics Department, Universität des Saarlandes, Saarbrücken, Germany

Abstract

The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In this review, we introduce recent developments in microfluidic-based space and time domain devices as well as discuss various designs integrated with multiple functions for sample preparation and detection. The development of isothermal nucleic acid amplification and digital PCR microfluidic devices within the last five years is also highlighted. Furthermore, we introduce various commercial microfluidic PCR devices.

Graphical abstract: Polymerase chain reaction in microfluidic devices

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Article information

DOI
https://doi.org/10.1039/C6LC00984K
Article type
Critical Review
Submitted
02 Aug 2016
Accepted
06 Sep 2016
First published
06 Sep 2016
This article is Open Access
Creative Commons BY license

Lab Chip, 2016,16, 3866-3884

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Polymerase chain reaction in microfluidic devices

C. D. Ahrberg, A. Manz and B. G. Chung, Lab Chip, 2016, 16, 3866 DOI: 10.1039/C6LC00984K

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