Abstract
Cytochrome c is an indispensable electron carrier in the mitochondrial respiratory chain and also an important mediator of the internal pathway triggering apoptosis. Mice with a complete deficiency of the Cycs gene encoding the somatic cytochrome c die during the embryogenesis. Using the technology of LoxP-cre-dependent tissue-specific recombination, we obtained some mouse strains with significantly reduced expression of cytochrome c in certain cell types (“conditional genetic knockdown”). This knockdown was achieved by abrogation of the normal splicing of the Cycs locus pre-mRNA due to an additional acceptor site inside the stop-cassette neor. Previously, we observed embryonic lethality in homozygous mice with the same knockdown of cytochrome c in all cells of the organism. In the present work we studied two novel mouse strains with conditional knockdown of the Cycs gene in T lymphocytes and macrophages. Somewhat surprisingly, the mice of these two strains under normal conditions were not phenotypically different from the wild-type mice, either on the whole organism level or on the level of activity of individual target cells. Thus, the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning.
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Original Russian Text © E. S. Shilov, I. V. Kislyakov, E. A. Gorshkova, R. V. Zvartsev, M. S. Drutskaya, I. A. Mufazalov, V. P. Skulachev, S. A. Nedospasov, 2014, published in Biokhimiya, 2014, Vol. 79, No. 12, pp. 1724–1736.
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Shilov, E.S., Kislyakov, I.V., Gorshkova, E.A. et al. Mouse lymphomyeloid cells can function with significantly decreased expression levels of cytochrome c . Biochemistry Moscow 79, 1412–1422 (2014). https://doi.org/10.1134/S0006297914120177
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DOI: https://doi.org/10.1134/S0006297914120177