Skip to main content
SpringerLink
Log in

Some peculiarities of steroid metabolism in transgenic Nicotiana tabacum plants bearing the CYP11A1 cDNA of cytochrome P450SCC from the bovine adrenal cortex

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

In the mitochondria of animal steroidogenic tissues, cytochrome P450SCC encoded by the CYP11A1 gene catalyzes the conversion of cholesterol into pregnenolone—the general precursor of all steroid hormones. In this work we study the steroid metabolism in transgenic tobacco plants carrying the CYP11A1 cDNA encoding cytochrome P450SCC from the bovine adrenal cortex. The transgenic plants under investigation markedly surpass the control wild-type plants by size and are characterized by a shortened period of vegetative growth (by rapid flowering); their leaves contain pregnenolone—the product of a reaction catalyzed by cytochrome P450SCC. The level of progesterone in transgenic tobacco leaves is higher than in the control plants of the wild type. The seeds of the transgenic plants contain less (24R)-brassinosteroids than the wild-type tobacco plants. The results obtained indicate that the synthesis of an active P450SCC cytochrome in transgenic Nicotiana tabacum plants has a profound effect on steroid metabolism and is responsible for the specific phenotypic features of transgenic plants bearing CYP11A1 cDNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

3β-HSD:

Δ5-hydroxysteroid dehydrogenase/Δ5-Δ4-ketosteroid isomerase

5αR:

steroid 5α-reductase

mRNA:

messenger RNA

RT-PCR:

reverse transcription coupled with polymerase chain reaction

SCC:

side-chain cleavage

References

  1. Bishop, G.J. and Yokota, T., Plant Cell Physiol., 2001, vol. 42, pp. 114–120.

    Article  CAS  PubMed  Google Scholar 

  2. Khripach, V.A., Zhabinskii, V.N., and de Groot, A., Brassinosteroids: A New Class of Plant Hormones, San Diego: Academic, 1999.

    Google Scholar 

  3. Payne, A.H. and Hales, D.B., Endocr. Rev., 2004, vol. 25, pp. 947–970.

    Article  CAS  PubMed  Google Scholar 

  4. Kreis, W., Hensel, A., and Stuhlemmer, U., Planta Med., 1998, vol. 64, pp. 491–499.

    Article  CAS  Google Scholar 

  5. Lindemann, P. and Luckner, M., Phytochemistry, 1997, vol. 46, pp. 507–513.

    Article  CAS  Google Scholar 

  6. Lindemann, P., Koch, A., Degenhardt, B., Hause, G., Grimm, B., and Papadopoulos, V., Plant Cell Physiol., 2004, vol. 45, pp. 723–733.

    Article  CAS  PubMed  Google Scholar 

  7. Papadopoulos, V., Endocr. Rev., 1993, vol. 14, pp. 222–240.

    CAS  PubMed  Google Scholar 

  8. Ohta, D. and Mizutani, M., Frontiers in Bioscience, 2004, vol. 9, pp. 1587–1597.

    Article  CAS  PubMed  Google Scholar 

  9. Takubo, K., Morikawa, T., Nonaka, Y., Mizutani, M., Takenaka, S., Takabe, K., Takahashi, M.A., and Ohta, D., Plant. Mol. Biol., 2003, vol. 52, pp. 817–830.

    Article  CAS  PubMed  Google Scholar 

  10. Grinberg, A.V., Hannemann, F., Schiffler, B., Muller, J., Heinemann, U., and Bernhardt, R., Proteins, 2000, vol. 40, pp. 590–612.

    Article  CAS  PubMed  Google Scholar 

  11. Piccioni, A., Dance, R., and Alban, C., J. Biol. Chem., 2003, vol. 278, pp. 24906–24975.

    Google Scholar 

  12. Li, J., Bisvas, G., Chao, A., Ruessel, D.W., and Chory, J., Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 3554–3559.

    Article  CAS  PubMed  Google Scholar 

  13. Rosati, F., Danza, G., Guarna, A., Cini, N., Racchi, M.L., and Serio, M., Endocrynology, 2003, vol. 144, pp. 220–229.

    Article  CAS  Google Scholar 

  14. Witt, M., Klonierung, Expression, Reinigung und Charakterisierungvon 3β-Hydroxysteroid-Dehydrogenasen aus Arabidipsis thaliana (L.) Heynhold: Dr. Rer. Nat., doctor rerum naturalium), Naturwissen-schaftlichen Fakultat i Biowissenschaftern der Martin-Luther-Universitat Halle-Wittenberg), 2008.

  15. Iino, M., Nomura, T., Tamaki, Y., Yamada, Y., Yoneyama, K., Takeuchi, Y., Mori, M., Asami, T., Nakano, T., and Yokota, T., Phytochemistry, 2007, vol. 68, pp. 1664–1673.

    Article  CAS  PubMed  Google Scholar 

  16. Xiao-Hua, Y., Zhi-Hong, X., and Hong-Wei, X., Plant Cell, 2005, vol. 17, pp. 116–131.

    Article  Google Scholar 

  17. RF Patent No. 2237717, 2004.

  18. Morohashi, K., Fujii-Kuriama, Y., Okada, Y., Sogawa, K., Hirose, T., Inayama, S., and Omura, T., Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 4647–4651.

    Article  CAS  PubMed  Google Scholar 

  19. John, M., A., John M.C., Asnley P., MacDonald R.J., Simpson E.R., Waterman M.R, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 5628–5632.

    Article  CAS  PubMed  Google Scholar 

  20. Matocha, M.F. and Waterman, M.R., J. Biol. Chem., 1985, vol. 260, pp. 12259–12265.

    CAS  PubMed  Google Scholar 

  21. Luzikov, V.N., Novikova, L.A., Spiridonova, V.A., Isaeva, L.V., Villan, Dzh., Kh’yugosson, M., and Glazer, E., Biokhimiya, 1994, vol. 59, pp. 1098–1101.

    CAS  Google Scholar 

  22. Shpakovskii, G.V., Spivak, S.G., Mazanik, S.N., and Akhrem, A.A., Materialy II-i Vsesoyuznoi planovootchetnoi konferentsii po napravleniyu “Gennaya i kletochnaya inzheneriya” (Pushchino-na-Oke, noyabr’–dekabr’ 1991 g) (Proc. II All-Union Conf. “Genetic and Cellular Engineering,” Pushchino-na-Oke, November–December 1991), 1992, p. 58.

  23. Graves, J.M.H. and Smith, W.K., Nature, 1967, vol. 214, pp. 1248–1249.

    Article  CAS  PubMed  Google Scholar 

  24. Seidel, S., Kreis, W., and Reinhard, E., Plant Cell Rep., 1990, vol. 8, pp. 621–624.

    Article  CAS  Google Scholar 

  25. Janeczko, A., Filek, W., Biesaga-Kocielniak, J., Marcinska, I., and Janeczko, Z., Plant Cell. Tissue and Organ Culture, 2003, vol. 72, pp. 147–151.

    Article  CAS  Google Scholar 

  26. Khripash, V.A., Sviridov, O.V., Pryadko, A.G., Litvinovskaya, R.R., Drach, S.V., Matveentsev, V.D., Novik, T.V., Mikhailopulo, K.I., Zhabinskii, V.N., Zavadskaya, M.I., Aver’kova, M.A., Drachenova, O.A., and Chashchina, N.M., Bioorg. Khim., 2007, vol. 33, pp. 371–378 [Russ. J. Bioorg. Chem. (Engl. Transl.), 2007, vol. 33, pp. 347–353].

    Google Scholar 

  27. Kolbe, A., Schneider, B., Porzel, A., Voigt, B., Krauss, G., and Adam, G., Phytochemistry, 1994, vol. 36, pp. 671–673.

    Article  CAS  Google Scholar 

  28. Saito, K., Noji, M., Ohmori, S., Imai, Y., and Murakoshi, I., Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 7041–7045.

    Article  CAS  PubMed  Google Scholar 

  29. Roger, P., Hellens, E., Edwards, A., Leyland, R., Bean, S., and Mullineaux, P., Plant. Mol. Biol., 2000, vol. 42, pp. 819–832.

    Article  Google Scholar 

  30. Plaschke, J., Ganal, M.W., and Roder, M.S., Theor. Appl. Genet., 1995, vol. 91, pp. 1001–1007.

    Article  CAS  Google Scholar 

  31. Southern, E.M., J. Mol. Biol., 1975, vol. 98, pp. 503–517.

    Article  CAS  PubMed  Google Scholar 

  32. Chomczynski, P. and Sacchi, N., Anal. Biochem., 1987, vol. 162, pp. 156–159.

    Article  CAS  PubMed  Google Scholar 

  33. Murashige, T. and Skoog, F., Physiol. Plant., 1962, vol. 15, pp. 473–497.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, ul. Kuprevicha 5/2, Minsk, 220141, Belarus

    S. G. Spivak, R. P. Litvinovskaya & S. V. Drach

  2. Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Belarus

    I. N. Berdichevets & N. A. Kartel

  3. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

    I. N. Berdichevets

  4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    G. V. Shpakovski

Authors
  1. S. G. Spivak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. N. Berdichevets
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. P. Litvinovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. V. Drach
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. A. Kartel
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. V. Shpakovski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to S. G. Spivak or G. V. Shpakovski.

Additional information

Original Russian Text © S.G. Spivak, I.N. Berdichevets, R.P. Litvinovskaya, S.V. Drach, N.A. Kartel, G.V. Shpakovski, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 2, pp. 241–250.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spivak, S.G., Berdichevets, I.N., Litvinovskaya, R.P. et al. Some peculiarities of steroid metabolism in transgenic Nicotiana tabacum plants bearing the CYP11A1 cDNA of cytochrome P450SCC from the bovine adrenal cortex. Russ J Bioorg Chem 36, 224–232 (2010). https://doi.org/10.1134/S1068162010020123

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162010020123

Key words

Search

Navigation