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Prolactin receptors in rat cholangiocytes: Regulation of level and isoform ratio is sex independent

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Abstract

The presence of prolactin receptor and peculiarities of its isoform expression in bile duct cells (cholangiocytes) differentially isolated from rat liver under different conditions were investigated in the present study. Normal cholangiocytes express prolactin receptor at relatively low level comparable to those of some prolactin-dependent tissues. Long receptor isoform is predominant in cholangiocytes but not in hepatocytes. The prolactin receptor level increases significantly under obstructive cholestasis due to evaluation of long and appearance of short isoforms. In rat cholangiocytes, unlike other tissues, the main positive regulators of prolactin receptor expression are cholestasis-induced factors instead of sex hormone and prolactin levels. Long isoform is predominant and induced primarily by cholestasis-induced factors.

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Abbreviations

CBDL:

common bile duct ligation

IHC:

immunohistochemical staining

PrlR:

prolactin receptor

RT-PCR:

polymerase chain reaction on the product of reverse transcription

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Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    R. L. Bogorad, T. Y. Ostroukhova, A. N. Orlova & O. V. Smirnova

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, 119991, Moscow, Russia

    P. M. Rubtsov

Authors
  1. R. L. Bogorad
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  2. T. Y. Ostroukhova
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  3. A. N. Orlova
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  4. P. M. Rubtsov
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  5. O. V. Smirnova
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Correspondence to R. L. Bogorad.

Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 2, pp. 229–236.

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Bogorad, R.L., Ostroukhova, T.Y., Orlova, A.N. et al. Prolactin receptors in rat cholangiocytes: Regulation of level and isoform ratio is sex independent. Biochemistry (Moscow) 71, 178–184 (2006). https://doi.org/10.1134/S0006297906020106

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  • DOI: https://doi.org/10.1134/S0006297906020106

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