No modality for selective heating of bone seems to have been reported in journal. Selective heating of bone by 332 kHz ultrasound wave was experimentally verified using bovine bone slice of 10mm thick covered by muscle layer of 10 mm thick and fat layer of 10 mm thick in the degassed water. The temperature rise, after 200 s of irradiation, at the center of the bone was 29.5°C that of muscle and fat was 9.0°C and 15.0°C respectively. The intensity of emitted ultrasound wave was approximately 100 W. Experiments were conducted using living rabbits resulting selective heating of the thighbone. Selective heating of bone may be of future clinical importance for treatment of bone cancer, rheumatism, bone fracture, and/or osteoporosis.