The absolute value of serum total cholesterol level always comes into question at the clinical study of hypertension. The author measured it by Broor's method under somevariable. From the results obtained, the following conclusions were reached.
1) Variation of coloring due to room-temperature and time.
The reaction of Lieberman-Buchard indicates that the time, in which it reaches to the highest coloring, is variable. The extent of light-absorbtion stabilizes for several minutes at the time of its highest coloring. This point is lower in summer and rather higher in winter.
2) Observational error.
The observational error on the 25 patient's investigation was measured as of 4.52% at maximum, 0% minimum and 2.14% on the average.
3) The recovery test.
The recovery test showed the high reliability of the method.