The authors quantitated the chemical components in the aqueous humor and the serum in eight anesthetized horses and thirty-one fractured horses to euthanasia. The chemical components of the serum were within normal range through the cases. The values for sodium, potassium, chloride and glucose in the aqueous humor were same as in the serum, while calcium, inorganic phosphate and total protein were less in the aqueous humor than in the serum. The chemical components and the enzyme activities in the aqueous humor were compared with the left and right eyes on the same horses. The values of chemical components of the aqueous humor were same in both eyes and the enzyme activities were low as regards LDH and GOT in the right eyes.