In order to assess the potential nephrotoxicity by various antibiotics with therapeutic concentration, effects of antibiotics administered to in vitro system were investigated in terms of protein synthesis and nucleic acid metabolism.
Kanamycin, streptomycin, gentamicin and colistin added to the incubation medium at the concentration of 10 μg/ml with kidney slices were demonstrated to inhibit the incorporation of orotic acid-6-¹⁴C into nuclear RNA.
The incorporation of orotic acid-6-¹⁴C into RNA of free polysomes prepared from human kidney slices was inhibited by kanamycin, streptomycin, gentamicin, colistin and chloramphenicol.
Significant reduction of the incorporation of L-leucine-¹⁴C into free polysome fraction was noticed by kanamycin, streptomycin and gentamicin added directly to cell free protein-synthesis system.
The incorporation of orotic acid-6-¹⁴C into RNA of bound polysome fraction prepared from human kidney slices was inhibited by kanamycin, streptomycin, gentamicin and colistin.
Inhibition of the incorporation of L-leucine-¹⁴C into bound polysome fraction was noticed when kanamycin, gentamicin and colistin were added directly in cell-free system.