Doppler sonography enables the assessment of blood flow and can be applied in the diagnosis of various lesions. Concomitant use of Doppler and conventional gray-scale sonography, for example, contributes to an excellent diagnosis with a higher accuracy in cervical lymph node metastases. Three organs are focused on in this study, the submandibular gland, the masseter muscle and the tongue, for which we have no or a relatively small amount of data obtained by Doppler sonography. For the submandibular gland, gustatory stimulation elicits both salivary secretion and blood flow to the gland through parasympathetic nervous control, and it has been reported that the secretion was well correlated to blood flow in animal experiments. We confirmed this correlation in humans. For the masseter muscle and tongue, there are no available Doppler sonography data and we determined the normal appearances of the four main arteries feeding the masseter muscle on Doppler sonograms in healthy volunteers. We speculated that flow velocity of the facial artery might be altered by local factors in the contracted masseter muscle as well as the general response in low-level static contraction of the masseter muscle. The deep lingual arteries were visible symmetrically on both sides of the lingual septum on the transverse Doppler sonogram of healthy volunteers. In patients with cancer of the tongue, characteristic Doppler sonographic features were the vasculature in and around the tumor and the enlarged deep lingual artery on the affected side. Although further studies are needed, Doppler sonography appears to be a promising diagnostic tool for these organs.