Development of a simple and rapid assay for methylase activity based on DNA hairpin probe and Sybr Green I

Yi Long; Xiaoming Zhou

doi:10.1117/12.918829

13 March 2012 Development of a simple and rapid assay for methylase activity based on DNA hairpin probe and Sybr Green I

Yi Long, Xiaoming Zhou

Author Affiliations +

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290C (2012) https://doi.org/10.1117/12.918829
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Methylase is vital for a large number of biological reactions. Here we developed a new method for DNA methylase activity analysis. In this paper, a DNA hairpin probe with a sequence of 5'-GATC-3' in the stem region was designed. The 5'-GATC-3' sequence was targeted by Dam MTase and was methylated. Subsequently, restriction enzyme Dpnl recognized the site and cut it. Then the haipin probe was transformed into three single stranded DNA. This enzymatic process can be monitored by the change of SYBR green I fluorescence. The current label free assay is an useful tool for DNA methylase activity analysis due to its simplicity, speedability, and low cost.

Citation Download Citation

Yi Long and Xiaoming Zhou "Development of a simple and rapid assay for methylase activity based on DNA hairpin probe and Sybr Green I", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290C (13 March 2012); https://doi.org/10.1117/12.918829

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available