Implementation of adaptive optics in fluorescent microscopy using wavefront sensing and correction

Oscar Azucena; Justin Crest; Jian Cao; William Sullivan; Peter Kner; Donald Gavel; Daren Dillon; Scot Olivier; Joel Kubby

doi:10.1117/12.846380

18 February 2010 Implementation of adaptive optics in fluorescent microscopy using wavefront sensing and correction

Oscar Azucena, Justin Crest, Jian Cao, William Sullivan, Peter Kner, Donald Gavel, Daren Dillon, Scot Olivier, Joel Kubby

Author Affiliations +

Proceedings Volume 7595, MEMS Adaptive Optics IV; 75950I (2010) https://doi.org/10.1117/12.846380
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

Adaptive optics (AO) improves the quality of astronomical imaging systems by using real time measurement of the turbulent medium in the optical path using a guide star (natural or artificial) as a point source reference beacon [1]. AO has also been applied to vision science to improve the view of the human eye. This paper will address our current research focused on the improvement of fluorescent microscopy for biological imaging utilizing current AO technology. A Shack-Hartmann wavefront sensor (SHWS) is used to measure the aberration introduced by a Drosophila Melanogaster embryo with an implanted 1 micron fluorescent bead that serves as a point source reference beacon. Previous measurements of the wavefront aberrations have found an average peak-to-valley and root-mean-square (RMS) wavefront error of 0.77 micrometers and 0.15 micrometers, respectively. Measurements of the Zernike coefficients indicated that the correction of the first 14 Zernike coefficients is sufficient to correct the aberrations we measured. Here we show that a MEMS deformable mirror with 3.5 microns of stroke and 140 actuators is sufficient to correct these aberrations. The design, assembly and initial results for the use of a MEMS deformable mirror, SHWS and implanted fluorescent reference beacon for wavefront correction are discussed.

Citation Download Citation

Oscar Azucena, Justin Crest, Jian Cao, William Sullivan, Peter Kner, Donald Gavel, Daren Dillon, Scot Olivier, and Joel Kubby "Implementation of adaptive optics in fluorescent microscopy using wavefront sensing and correction", Proc. SPIE 7595, MEMS Adaptive Optics IV, 75950I (18 February 2010); https://doi.org/10.1117/12.846380

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available