Chemiluminescent solid lipid nanoparticles (SLN) and interations with intact skin

Jennifer Breidenich; Julia Patrone; Lisa Kelly; Jason Benkoski; Huong Le; Jennifer Sample

doi:10.1117/12.826397

24 August 2009 Chemiluminescent solid lipid nanoparticles (SLN) and interations with intact skin

Jennifer Breidenich, Julia Patrone, Lisa Kelly, Jason Benkoski, Huong Le, Jennifer Sample

Proceedings Volume 7397, Biosensing II; 73970L (2009) https://doi.org/10.1117/12.826397
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

We report the synthesis and characterization of a novel nanoparticle formulation designed for skin penetration for the purpose of skin imaging. Solid lipid nanoparticles (SLNs), a drug delivery vehicle, were used as the matrix for targeted delivery of peroxide-sensitive chemiluminescent compounds to the epidermis. Luminol and oxalate were chosen as the chemiluminescent test systems, and a formulation was determined based upon non-toxic components, lotion-like properties, and longevity/visibility of a chemiluminescent signal. The luminescence lifetime was extended in the lipid formulation in comparison to the chemiluminescent system in solution. When applied to porcine skin, our formulation remained detectable relative to negative and positive controls. Initial MTT toxicity testing using HepG2 cells have indicated that this formulation is relatively non-toxic. This formulation could be used to image native peroxides present in tissue that may be indicative of skin disease.

Citation Download Citation

Jennifer Breidenich, Julia Patrone, Lisa Kelly, Jason Benkoski, Huong Le, and Jennifer Sample "Chemiluminescent solid lipid nanoparticles (SLN) and interations with intact skin", Proc. SPIE 7397, Biosensing II, 73970L (24 August 2009); https://doi.org/10.1117/12.826397

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available