SPDM: single molecule superresolution of cellular nanostructures

Rainer Kaufmann; Paul Lemmer; Manuel Gunkel; Yanina Weiland; Patrick Müller; Michael Hausmann; David Baddeley; Roman Amberger; Christoph Cremer

doi:10.1117/12.809109

24 February 2009 SPDM: single molecule superresolution of cellular nanostructures

Rainer Kaufmann, Paul Lemmer, Manuel Gunkel, Yanina Weiland, Patrick Müller, Michael Hausmann, David Baddeley, Roman Amberger, Christoph Cremer

Author Affiliations +

Proceedings Volume 7185, Single Molecule Spectroscopy and Imaging II; 71850J (2009) https://doi.org/10.1117/12.809109
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Novel methods of visible light microscopy have overcome the limits of resolution hitherto thought to be insurmountable. The localization microscopy technique presented here based on the principles of Spectral Precision Distance Microscopy (SPDM) with conventional fluorophores under special physical conditions allows to measure the spatial distribution of single fluorescence labeled molecules in entire cells with macromolecular precision which is comparable to a macromolecular effective optical resolution. Based on detection of single molecules, in a novel combination of SPDM and Spatially Modulated Illumination (SMI) microscopy, a lateral (2D) effective optical resolution of cellular nanostructures around 10 - 20 nm (about 1/50^th of the exciting wavelength) and a three dimensional (3D) effective optical resolution in the range of 40 - 50 nm are achieved.

Citation Download Citation

Rainer Kaufmann, Paul Lemmer, Manuel Gunkel, Yanina Weiland, Patrick Müller, Michael Hausmann, David Baddeley, Roman Amberger, and Christoph Cremer "SPDM: single molecule superresolution of cellular nanostructures", Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850J (24 February 2009); https://doi.org/10.1117/12.809109

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