Effects of isoflurane on measurement of fluorescence spectra and CLSM imaging in Acetabularia acetabulum

WenLi Chen D.D.S.; Zhou Quan; Da Xing

doi:10.1117/12.741359

10 May 2007 Effects of isoflurane on measurement of fluorescence spectra and CLSM imaging in Acetabularia acetabulum

WenLi Chen D.D.S., Zhou Quan, Da Xing

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65340Q (2007) https://doi.org/10.1117/12.741359
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

The volatile halogenated methyl ethyl ether is used as anesthetic to inhibit actin-based dynamics directly or indirectly in animal cells. In plant cells, most intracellular movements are related with actin pathways too. We utilized isoflurane to study the dynamic choloroplast organization in unicellular baby and adult alga Acetabularia acetabulum. Fluorescence spectra were measured and choloroplast movements were recorded by confocal laser scanning microscope (CLSM) imaging in in Acetabularia acetabulum. Isoflurane was effective in the unicellular baby and adult organisms and showed time- dependent actin-inhibition patterns. Acetabularia acetabulum cells were treated for different times with isoflurane saturated solutions in artificial seawater (it was defined to be 100% isoflurane). The intensity of fluorescence at 680nm and 730nm were progressively decreased at 100% isoflurane. It was remarkable difference between fluorescence spectra of baby and adult Acetabularia were inhibited by isoflurance, adult Acetabularia cells showed more sensitive. Whereas the choloroplast in Acetabularia acetabulum was commendably imaged by CLSM at 20 and 40 zoom.

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WenLi Chen D.D.S., Zhou Quan, and Da Xing "Effects of isoflurane on measurement of fluorescence spectra and CLSM imaging in Acetabularia acetabulum", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65340Q (10 May 2007); https://doi.org/10.1117/12.741359

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KEYWORDS

Luminescence

Microscopes

Laser scanners

Confocal microscopy

Zoom lenses

Life sciences

Picosecond phenomena

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