High sensitivity detection of bacteria by surface plasmon resonance enhanced common path interferometry

Charles Greef; Viatcheslav Petropavlovskikh; Oyvind Nilsen; Bilge Hacioglu; Boris Khattatov; John Hall

doi:10.1117/12.719409

26 April 2007 High sensitivity detection of bacteria by surface plasmon resonance enhanced common path interferometry

Charles Greef, Viatcheslav Petropavlovskikh, Oyvind Nilsen, Bilge Hacioglu, Boris Khattatov, John Hall

Proceedings Volume 6554, Chemical and Biological Sensing VIII; 65540L (2007) https://doi.org/10.1117/12.719409
Event: Defense and Security Symposium, 2007, Orlando, Florida, United States

Abstract

Real time monitoring of biowarfare agents (BWA) for military and civilian protection remains a high priority for homeland security and battlefield readiness. Available devices have adequate sensitivity, but the detection modules have limited periods of deployment, require frequent maintenance, employ single-use disposable components, and have limited multiplexing capability. Surface Plasmon Resonance enhanced Common Path Interferometry (SPR-CPI) is a label-free, high sensitivity biomolecular interaction measurement technology that allows multiplexed real-time measurement of biowarfare agents, including small molecules, proteins, and microbes. The technology permits continuous operation in a field-deployable detection module of an integrated BWA monitoring system. SPR-CPI measures difference in phase shift of polarized light reflected from the transducer interface caused by changes in refractive index induced by biomolecular interactions. The measurement is performed on a discrete 2-dimensional area functionalized with biomolecule capture reagents in a microarray format, allowing simultaneous measurement of up to 100 separate analytes. Output consists of simultaneous voltage measurements proportional to the phase differences resulting from the refractive index changes and is automatically processed and displayed graphically or delivered to a decision making algorithm. This enables a fully automatic field-deployable detection system capable of integration into existing modular BWA detection systems. Proof-of-concept experiments on surrogate models of anticipated BWA threats have demonstrated utility. Efforts are in progress for full development and deployment of the device.

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Charles Greef, Viatcheslav Petropavlovskikh, Oyvind Nilsen, Bilge Hacioglu, Boris Khattatov, and John Hall "High sensitivity detection of bacteria by surface plasmon resonance enhanced common path interferometry", Proc. SPIE 6554, Chemical and Biological Sensing VIII, 65540L (26 April 2007); https://doi.org/10.1117/12.719409

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KEYWORDS

Resonance enhancement

Interferometry

Refractive index

Surface plasmons

Bacteria

Multiplexing

System integration

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