Epidermal growth factor signaling studied using multidimensional single molecule fluorescence microscopy

Stephen E. D. Webb; Selene K. Roberts; Sarah R. Needham; Daniel J. Rolfe; Chris J. Tynan; Marisa Martin-Fernandez

doi:10.1117/12.701417

19 February 2007 Epidermal growth factor signaling studied using multidimensional single molecule fluorescence microscopy

Stephen E. D. Webb, Selene K. Roberts, Sarah R. Needham, Daniel J. Rolfe, Chris J. Tynan, Marisa Martin-Fernandez

Author Affiliations +

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410E (2007) https://doi.org/10.1117/12.701417
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Quantification of the oligomerisation state and conformational changes of the epidermal growth factor receptor in its constitutive and active states are critical to fully understand their signal transduction pathway. We show the ability of single-molecule fluorescence microscopy to determine this information and its implementation by combining multidimensional emission optics with total internal reflection fluorescence microscopy. Importantly, the signalling process is analysed in live cells under physiological conditions.

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Stephen E. D. Webb, Selene K. Roberts, Sarah R. Needham, Daniel J. Rolfe, Chris J. Tynan, and Marisa Martin-Fernandez "Epidermal growth factor signaling studied using multidimensional single molecule fluorescence microscopy", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410E (19 February 2007); https://doi.org/10.1117/12.701417

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KEYWORDS

Luminescence

Molecules

Microscopy

Receptors

Polarization

Microscopes

Signal processing

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