Imaging of human tissue with phase-resolved polarization-sensitive optical coherence tomography based on transversal scanning

Michael Pircher; Erich Goetzinger; Rainer Leitgeb; Adolf F. Fercher; Christoph K. Hitzenberger

doi:10.1117/12.530871

1 July 2004 Imaging of human tissue with phase-resolved polarization-sensitive optical coherence tomography based on transversal scanning

Michael Pircher, Erich Goetzinger, Rainer Leitgeb, Adolf F. Fercher, Christoph K. Hitzenberger

Author Affiliations +

Proceedings Volume 5316, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII; (2004) https://doi.org/10.1117/12.530871
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We introduce a novel OCT-technique to measure backscattered intensity and birefringence properties (retardation and fast axis orientation) of human tissue. The experimental setup consists of a Mach Zehnder interferometer. A polarizing beam splitter placed after each interferometer exit allows a dual balanced polarization sensitive detection of the backscattered light intensity from the sample. Two acousto optic modulators (AOM's) placed in the reference arm introduce a frequency shift to the reference beam causing a beat frequency on the detection unit whenever light beams from the sample and reference arm are within the coherence gate. A translation stage placed in the reference arm allows a change in the reference arm length. Image acquisition is performed by transversal scanning of the sample at different reference arm mirror positions (corresponding to different depth positions in the sample). Two lock-in amplifiers set to the beat frequency are used to obtain real and imaginary part of each interferometric signal. This setup allows a phase resolved and polarization sensitive acquisition of the backscattered light from the sample. We demonstrate this method on human tissue in vivo and present, to the best of our knowledge, the first OCT images that are directly related to the fast axis orientation of human tissue in vivo.

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Michael Pircher, Erich Goetzinger, Rainer Leitgeb, Adolf F. Fercher, and Christoph K. Hitzenberger "Imaging of human tissue with phase-resolved polarization-sensitive optical coherence tomography based on transversal scanning", Proc. SPIE 5316, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII, (1 July 2004); https://doi.org/10.1117/12.530871

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