Computed tomography of the periphery blood lymphocytes and erythrocytes

Elena A. Streletskaya; Julia K. Novoderzhkina; Gennady N. Vishnyakov; Gennady G. Levin; Gennady I. Kozinets

doi:10.1117/12.413804

19 January 2001 Computed tomography of the periphery blood lymphocytes and erythrocytes

Elena A. Streletskaya, Julia K. Novoderzhkina, Gennady N. Vishnyakov, Gennady G. Levin, Gennady I. Kozinets

Proceedings Volume 4158, Biomonitoring and Endoscopy Technologies; (2001) https://doi.org/10.1117/12.413804
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Many biological objects, including blood cells, are optically transparent. They don't absorb light and only change optical path length by variations of object's thickness or its refractive index variations. The phase shifting interferometry is used for the visualization of the phase information and the obtaining quantitative properties of cells. Cells are fixed by glutaraldehyde and mounted into immersion liquid for influences of refraction reduction. Blood cell's interferogram contains the information about space distribution refractive index. The phase-shift method was used for its reconstruction. This method includes record of four interferograms used different values of phase-shift of the reference wave. Many blood cells' interferograms are obtained (lymphocytes and erythrocytes). The shape, size and density of cells are measurement. We suppose that these date will be ones of the early diagnostically criteria of hematological diseases. Experimental results for interferometric microscopy and tomography of blood cells are presented.

Citation Download Citation

Elena A. Streletskaya, Julia K. Novoderzhkina, Gennady N. Vishnyakov, Gennady G. Levin, and Gennady I. Kozinets "Computed tomography of the periphery blood lymphocytes and erythrocytes", Proc. SPIE 4158, Biomonitoring and Endoscopy Technologies, (19 January 2001); https://doi.org/10.1117/12.413804

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