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13 March 2024 Polar-SIM: super-resolution structured illumination microscopy in XYZλθT dimensions
Peng Xi, He Sun
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Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530B (2024) https://doi.org/10.1117/12.3008475
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
To further extend the capability of super-resolution in XYZλθT 6 dimensions, we developed polarized structured illumination microscopy (Polar-SIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular (XYZθ) hyperspace. Further, the multi-wavelength excitation enables study of the dynamic interactions of subcellular organelles. We demonstrate the application of Polar-SIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. With the modulation of the incident angle, we can generate TIRF- or HiLo- illumiation for Polar-SIM. Also, for thick specimen, the structured illumination can be used with lower frequency to generate grid confocal, enabling the optical sectioning capability.
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Peng Xi and He Sun "Polar-SIM: super-resolution structured illumination microscopy in XYZλθT dimensions", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530B (13 March 2024); https://doi.org/10.1117/12.3008475
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KEYWORDS
Light sources and illumination
Super resolution
Microscopy
Modulation
Point spread functions
Molecules
Neurons
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