Remote focusing for 2-photon microscopy to follow action potential propagation transmurally in acute rabbit cardiac slices

Giedrė Astrauskaitė; Sharika Mohanan; Lewis Williamson; Erin Boland; Eline Huethorst; Ryo Kinegawa; Godfrey Smith; Caroline Müllenbroich

doi:10.1117/12.3002956

12 March 2024 Remote focusing for 2-photon microscopy to follow action potential propagation transmurally in acute rabbit cardiac slices

Giedrė Astrauskaitė, Sharika Mohanan, Lewis Williamson, Erin Boland, Eline Huethorst, Ryo Kinegawa, Godfrey Smith, Caroline Müllenbroich

Author Affiliations +

Proceedings Volume 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; 128470P (2024) https://doi.org/10.1117/12.3002956
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

In the heart, action potentials travel through the tissue to orchestrate muscle contraction and make the organ an efficient pump. Scar tissues caused by myocardial infarction impairs electrical conduction. In the rabbit heart, action potentials propagate from the endocardium to the epicardium with a conduction velocity of 30 cm/s. Therefore, a rapid vertical scan is necessary to observe this transmural cardiac conduction at cellular resolution. Here we present an implementation of a versatile remote focusing module, compatible with retrofitting to commercial two-photon microscopes and capable of 0.3 kHz rate axial scanning over the range of 100 μm in cardiac tissue without disturbing the sample or the sample objective. We discuss the necessary optimization to compensate for pulse broadening, power losses and optical aberrations. We demonstrate fast imaging of cardiac cell structure in functionally viable rabbit ventricular slice model. We will apply this system to resolve cardiac electrical signal propagation transmurally in healthy and infarcted hearts.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Giedrė Astrauskaitė, Sharika Mohanan, Lewis Williamson, Erin Boland, Eline Huethorst, Ryo Kinegawa, Godfrey Smith, and Caroline Müllenbroich "Remote focusing for 2-photon microscopy to follow action potential propagation transmurally in acute rabbit cardiac slices", Proc. SPIE 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, 128470P (12 March 2024); https://doi.org/10.1117/12.3002956

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