CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 12851 > Article
Presentation + Paper
12 March 2024 Direct access to optical aberration information in fluorescence laser scanning microscopy using detector arrays
Francesco Fersini, Alessandro Zunino, Pietro Morerio, Alessio Del Bue, Martin J. Booth, Giuseppe Vicidomini
Author Affiliations +
Proceedings Volume 12851, Adaptive Optics and Wavefront Control for Biological Systems X; 1285102 (2024) https://doi.org/10.1117/12.3000914
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
In the recent years, numerous adaptive optics techniques have emerged to address optical aberrations in fluorescence microscopy imaging. However, many existing methods involve complex hardware implementations or lengthy iterative algorithms that may induce photo-damage to the sample. Our study proposes an innovative approach centered around a novel detector array capable of potentially capturing the probed sample in a single acquisition. Our solution is gentle on the sample and applicable to any laser scanning microscope equipped with a detector array. We demonstrate that the multi-dimensional dataset obtained using the detector array inherently encodes information about optical aberrations. Finally, we propose a convolutional neural network approach to decode these optical aberrations in real-time and with high accuracy, establishing the foundation for a new class of adaptive optics laser-scanning microscopy methods.
Conference Presentation
(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Francesco Fersini, Alessandro Zunino, Pietro Morerio, Alessio Del Bue, Martin J. Booth, and Giuseppe Vicidomini "Direct access to optical aberration information in fluorescence laser scanning microscopy using detector arrays", Proc. SPIE 12851, Adaptive Optics and Wavefront Control for Biological Systems X, 1285102 (12 March 2024); https://doi.org/10.1117/12.3000914
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 8 PAGES + PRESENTATION
DOWNLOAD PAPER SAVE TO MY LIBRARY
WATCH
PRESENTATION
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Optical aberrations
Detector arrays
Adaptive optics
Computer simulations
Image processing
Microscopes
Confocal microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top