A flexible and controllable condenser device based on programmable LCD for multi-modal imaging

Qiulong Xu; Yao Fan; Chao Zuo

doi:10.1117/12.3000754

18 December 2023 A flexible and controllable condenser device based on programmable LCD for multi-modal imaging

Qiulong Xu, Yao Fan, Chao Zuo

Proceedings Volume 12967, AOPC 2023: Computing Imaging Technology; 1296704 (2023) https://doi.org/10.1117/12.3000754
Event: Applied Optics and Photonics China 2023 (AOPC2023), 2023, Beijing, China

Abstract

The rise of “computational optics” provides a new way for label-free microscopic imaging. Programmable illumination modulation devices [light emitting diode (LED), liquid-crystal-display (LCD), or spatial light modulator (SLM)] have been introduced into light microscopes to give them multimodal imaging capabilities. Nevertheless, due to the discontinuity of the LED array and the high light loss of the LCD, the existing illumination modulation scheme still needs to be optimized. In this paper, we report a programmable illumination modulation condenser for multi-modal microscopic imaging. By placing a high transmittance LCD inside a condenser, this device enables flexible modulation of the illumination light wavelength and angle without altering the existing optical layout. The high transmittance LCD greatly increases the transmittance of illumination light, which is further converged on the sample by the condenser, resulting in higher illumination intensity to produce high contrast images. In addition, the programmable condenser can be used as a flexible add-on module compatible with any commercial microscope to extend the imaging modality of conventional bright-field microscopes. Ultimately, we validated the accuracy and dependability of the programmable illumination modulation condenser by imaging spongy germ cells.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Qiulong Xu, Yao Fan, and Chao Zuo "A flexible and controllable condenser device based on programmable LCD for multi-modal imaging", Proc. SPIE 12967, AOPC 2023: Computing Imaging Technology, 1296704 (18 December 2023); https://doi.org/10.1117/12.3000754

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KEYWORDS

Light sources and illumination

Microscopes

LCDs

Multimodal imaging

Modulation

Biological imaging

Imaging systems

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