Sequential counting of single molecules in a microcapillary

Christoph Zander; Karl-Heinz Drexhage

doi:10.1117/12.273567

7 May 1997 Sequential counting of single molecules in a microcapillary

Christoph Zander, Karl-Heinz Drexhage

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273567
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We studied photon bursts of individual rhodamine 6G molecules in ethylene glycol flowing through a microcapillary (diameter 1 micrometers ). Excitation was provided by an argon ion laser ((lambda) equals 514.5 nm). The laser beam was coupled into a confocal microscope by a glass plate and focussed onto the sample by an oil-immersion objective (magnification 100X). Fluorescence was collected by the same objective and detected by a photomultiplier. In order to reject out-of-focus signals a pinhole (diameter 100 micrometers ) was placed in the image plane. Several filters were used to suppress laser stray light and Raman scattered photons. With the setup described sequential counting of individual molecules is possible.

Citation Download Citation

Christoph Zander and Karl-Heinz Drexhage "Sequential counting of single molecules in a microcapillary", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273567

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