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15 March 2023 The Phasor approach to FLIM: Metabolic imaging reveals how P53 and REV3L regulate energy metabolism in lung carcinoma cells (Conference Presentation)
Michelle A. Digman
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Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC1238402 (2023) https://doi.org/10.1117/12.2653032
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
The majority of cancer cells have high frequencies of DNA damage response defects which results in a deficient repair mechanism which can give rise to oncogenes that regulate cellular metabolism. In this project, the effects of tumor suppressor protein p53 and translesional synthesis protein REV3L are studied to relate DNA damage signaling and repair to cellular metabolism by using the fluorescence lifetime of the metabolic coenzyme NADH. Our results show that restoring function to p53 and silencing REV3L simultaneously suppressed the cancerous metabolic phenotype and resulted in the greatest amount of cancer cell death
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Michelle A. Digman "The Phasor approach to FLIM: Metabolic imaging reveals how P53 and REV3L regulate energy metabolism in lung carcinoma cells (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC1238402 (15 March 2023); https://doi.org/10.1117/12.2653032
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KEYWORDS
Fluorescence lifetime imaging
Lung
Mode conditioning cables
Cancer
Lung cancer
Cell death
Resistance
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