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6 March 2023 A microfluidic chip with immobilization chambers for cardiac organoid imaging
Khashayar Moshksayan, Anirudha Harihara, Sudip Mondal, Evan Hegarty, Janet Zoldan, Adela Ben-Yakar
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Proceedings Volume 12374, Microfluidics, BioMEMS, and Medical Microsystems XXI; 1237404 (2023) https://doi.org/10.1117/12.2647888
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Organoids are three-dimensional structures of self-assembled cell aggregates that mimic anatomical features of in vivo organs and, therefore, can serve as in vitro miniaturized organ models for drug testing. The most effective way of studying drug toxicity and efficacy requires fast-paced and high-resolution imaging of organoids which is possible by first immobilizing the organoids. However, suitable platforms that are conducive to high-throughput imaging and can fully immobilize the organoids are missing. We propose a unique microfluidic device capable of hydrogel-free immobilization of cardiac organoids in predetermined locations for viability staining and calcium-transients imaging without compromising their morphology and behavior. In doing so, we treated cardiac organoids with Doxorubicin (DOX), and successfully immobilized and imaged them within our device to evaluate organoid viability upon treatment.
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Khashayar Moshksayan, Anirudha Harihara, Sudip Mondal, Evan Hegarty, Janet Zoldan, and Adela Ben-Yakar "A microfluidic chip with immobilization chambers for cardiac organoid imaging", Proc. SPIE 12374, Microfluidics, BioMEMS, and Medical Microsystems XXI, 1237404 (6 March 2023); https://doi.org/10.1117/12.2647888
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KEYWORDS
Microfluidics
Fluorescence imaging
Design and modelling
Toxicity
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