Dynamic tracking of water influx and efflux across cellular membranes using quantitative phase imaging (Conference Presentation)

Zachary A. Steelman; Stacey Martens; Jennifer Tran; Anna Sedelnikova; Allen Kiester; Bennett Ibey; Joel N. Bixler

doi:10.1117/12.2647560

16 March 2023 Dynamic tracking of water influx and efflux across cellular membranes using quantitative phase imaging (Conference Presentation)

Zachary A. Steelman, Stacey Martens, Jennifer Tran, Anna Sedelnikova, Allen Kiester, Bennett Ibey, Joel N. Bixler

Author Affiliations +

Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC123910D (2023) https://doi.org/10.1117/12.2647560
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Water is the most basic requirement of life, acting as a medium of exchange, transport, homeostasis and diffusion. We present a technique which leverages quantitative phase imaging (QPI) and a two-component mixture model to quantify, in absolute terms, the intracellular water fraction (by mass and volume) as well as the intracellular water volume in femtoliters. We demonstrate our technique by applying a single microsecond-duration electric pulse, and observe water influx in physiological buffer, water efflux in hyperosmotic buffer, and no movement across the membrane of synthetic vesicles. Dose response curves and channel blockers are employed to better understand underlying phenomena.

Conference Presentation

Citation Download Citation

Zachary A. Steelman, Stacey Martens, Jennifer Tran, Anna Sedelnikova, Allen Kiester, Bennett Ibey, and Joel N. Bixler "Dynamic tracking of water influx and efflux across cellular membranes using quantitative phase imaging (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC123910D (16 March 2023); https://doi.org/10.1117/12.2647560

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