Morphological changes in rat lung tissue during inhalation of e-cigarette liquid aerosol

Alla Bucharskaya; Dmitry Mudrak; Irina Yanina; Nikita Navolokin; Alexandr Polozhenkov; Elina Genina; Yury Kistenev; Valery Tuchin

doi:10.1117/12.2627915

29 April 2022 Morphological changes in rat lung tissue during inhalation of e-cigarette liquid aerosol

Alla Bucharskaya, Dmitry Mudrak, Irina Yanina, Nikita Navolokin, Alexandr Polozhenkov, Elina Genina, Yury Kistenev, Valery Tuchin

Author Affiliations +

Proceedings Volume 12192, Optical Technologies for Biology and Medicine; 1219210 (2022) https://doi.org/10.1117/12.2627915
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

For potential use as a diagnostic test, morphological changes in the lung tissue of rats after inhalationof e-cigarette liquid based on polypropylene glycol and glycerol were studied ex vivo and in vivo. Lungs of Wistar rats placed for 1 hour in ecigarette liquid were used in the ex vivo study. The in vivo study was performed on 12 mature male Wistar rats. The animals were divided into two groups - experimental and control, 6 rats in each. Rats of the experimental group were placed in aerosol administration chamber and exposed to an aerosol of nicotine-free liquid for electronic cigarettes. Rats of the control group were intact. The animals were removed from the experiment one hour after inhalation, lung tissue samples were examined ex vivo using optical coherence tomography (OCT) and lung tissue spectroscopy. After manipulations, lung samples were fixed in 10% formalin solution and embedded in paraffin. Then histological sections were made according to the conventional methods, followed by hematoxylin and eosin staining. Comparative analysis of B-scans ex vivo of lung tissue samples placed in e-cigarette liquid and control samples showed the presence of a luminescent effect on lung tissue in the studied solution, as alveoli are filled with the solution and scattering is reduced. The structure of the tissue differs from the control samples. B-scan in vivo studies are characterized by the presence of voids of rather large size. Histological examination of ex vivo and in vivo specimens noted morphological changes in lung tissue structure corresponding to the changes observed in OCT.

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Alla Bucharskaya, Dmitry Mudrak, Irina Yanina, Nikita Navolokin, Alexandr Polozhenkov, Elina Genina, Yury Kistenev, and Valery Tuchin "Morphological changes in rat lung tissue during inhalation of e-cigarette liquid aerosol", Proc. SPIE 12192, Optical Technologies for Biology and Medicine, 1219210 (29 April 2022); https://doi.org/10.1117/12.2627915

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KEYWORDS

Lung

Tissues

Liquids

In vivo imaging

Tissue optics

Optical coherence tomography

Scattering

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