High-resolution deep brain imaging using adaptive optics three-photon microscopy

Robert Prevedel

doi:10.1117/12.2614975

2 March 2022 High-resolution deep brain imaging using adaptive optics three-photon microscopy

Robert Prevedel

Proceedings Volume PC11969, Adaptive Optics and Wavefront Control for Biological Systems VIII; PC1196904 (2022) https://doi.org/10.1117/12.2614975
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Multiphoton microscopy has become a powerful tool for imaging the intact mammalian brain, however, tissue scattering, optical aberrations and motion artifacts degrade the imaging performance at depth. Here we developed a minimally invasive intravital imaging methodology based on three-photon excitation, indirect adaptive optics (AO) and active electrocardiogram gating to advance deep-tissue imaging. We demonstrate near-diffraction-limited imaging of deep cortical spines and (sub-)cortical dendrites up to a depth of 1.4 mm in the mouse hippocampus, as well as deep-layer calcium imaging of astrocytes that reside in the highly scattering corpus callosum.

Conference Presentation

Citation Download Citation

Robert Prevedel "High-resolution deep brain imaging using adaptive optics three-photon microscopy", Proc. SPIE PC11969, Adaptive Optics and Wavefront Control for Biological Systems VIII, PC1196904 (2 March 2022); https://doi.org/10.1117/12.2614975

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