Three dimensional tumor spheroid drug response evaluation using OCT based tissue viability evaluation method

Ibrahim Abd El-Sadek; Arata Miyazawa; Tomoko Mori; Larina Tzu-Wei Shen; Shuichi Makita; Pradipta Mukherjee; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.2612128

7 March 2022 Three dimensional tumor spheroid drug response evaluation using OCT based tissue viability evaluation method

Ibrahim Abd El-Sadek, Arata Miyazawa, Tomoko Mori, Larina Tzu-Wei Shen, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume 11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI; 119480A (2022) https://doi.org/10.1117/12.2612128
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a 3-D non-invasive OCT-based tissue dynamics imaging method to evaluate the tumor spheroid drug response. Our method depends on newly developed 3-D scanning protocol, which acquires the volumetric tomography in 52.4 s. The scanning protocol repeats raster scanning 32 times at each location in the tissue in 6.55 s. The tissue sub-cellular motion/viability is quantified by analyzing the OCT time sequence using our developed algorithms including “logarithmic intensity variance algorithm (LIV)” and “late OCT correlation decay speed (OCDS_l)”. The capability of our method has been investigated by evaluating the response of the human originated breast cancer (MCF-7) and colon cancer (HT-29) spheroids to anti-cancer drugs. The tissue viability alterations induced by the drug applications have been successfully visualized and quantified.

Conference Presentation

Citation Download Citation

Ibrahim Abd El-Sadek, Arata Miyazawa, Tomoko Mori, Larina Tzu-Wei Shen, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, and Yoshiaki Yasuno "Three dimensional tumor spheroid drug response evaluation using OCT based tissue viability evaluation method", Proc. SPIE 11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, 119480A (7 March 2022); https://doi.org/10.1117/12.2612128

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