Waving away from the poison: recovery of the nuclear structure after chemotropic response in roots against poison

Aditya Kurup; Aiswarya K. S.; Bejoy Manoj; Rinsa Salahudeen Rafeeka; Mayanglambam Suheshkumar Singh

doi:10.1117/12.2609961

3 March 2022 Waving away from the poison: recovery of the nuclear structure after chemotropic response in roots against poison

Aditya Kurup, Aiswarya K. S., Bejoy Manoj, Rinsa Salahudeen Rafeeka, Mayanglambam Suheshkumar Singh

Author Affiliations +

Proceedings Volume 11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX; 1196409 (2022) https://doi.org/10.1117/12.2609961
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The improper disposal of textile dyes like Rhodamine B into nearby water bodies and land areas affects living organisms nearby. Here, we explore the effects of rhodamine absorption through the soil into the plant root and how variation in rhodamine concentration affects the growth and development of the plant. The developmental defects over time are verified by nuclear staining the plant cells. Furthermore, we hypothesis that the chemotropic effect seen in plants guides the root away from rhodamine B high concentration regions to low concentration regions. This will in turn help the plant recover from the injury. These minute structural variations are analyzed with the help of our in-house developed dual-arm multi-level magnification light-sheet microscopy (DMx-LSFM) system. The microscope consists of an automated (translating and rotating) sample holding stage, which helps to observe nearby regions by bringing the region of interest into a plane of focus without disturbing the sample.

Conference Presentation

Citation Download Citation

Aditya Kurup, Aiswarya K. S., Bejoy Manoj, Rinsa Salahudeen Rafeeka, and Mayanglambam Suheshkumar Singh "Waving away from the poison: recovery of the nuclear structure after chemotropic response in roots against poison", Proc. SPIE 11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, 1196409 (3 March 2022); https://doi.org/10.1117/12.2609961

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