Characterization of a large gated SPAD array for in vivo fluorescence lifetime imaging of drug target engagement

Jason T. Smith; Alena Rudkouskaya; Shan Gao; Arin Ulku; Claudio Bruschini; Edoardo Charbon; Shimon Weiss; Margarida Barroso; Xavier Intes; Xavier Michalet

doi:10.1117/12.2609888

7 March 2022 Characterization of a large gated SPAD array for in vivo fluorescence lifetime imaging of drug target engagement

Jason T. Smith, Alena Rudkouskaya, Shan Gao, Arin Ulku, Claudio Bruschini, Edoardo Charbon, Shimon Weiss, Margarida Barroso, Xavier Intes, Xavier Michalet

Author Affiliations +

Proceedings Volume PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX; PC1196408 (2022) https://doi.org/10.1117/12.2609888
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Near-Infrared wide-field Fluorescence Lifetime Imaging (FLI) has become an increasingly popular method due to its unique specificity in sensing the cellular micro-environment and/or protein-protein interactions via FRET, but the approach is still challenging due to inefficient detection modules. Here, we report on the characterization of a large gated SPAD array, SwissSPAD2, towards in vivo preclinical imaging of FLI-FRET. Fluorescence decay fitting as well as phasor analysis are used to demonstrate the ability of SwissSPAD2 to accurately quantify short lifetimes and associated lifetime parameters in both in vitro and in vivo experiments, in full agreement with gated ICCD measurements.

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Jason T. Smith, Alena Rudkouskaya, Shan Gao, Arin Ulku, Claudio Bruschini, Edoardo Charbon, Shimon Weiss, Margarida Barroso, Xavier Intes, and Xavier Michalet "Characterization of a large gated SPAD array for in vivo fluorescence lifetime imaging of drug target engagement", Proc. SPIE PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, PC1196408 (7 March 2022); https://doi.org/10.1117/12.2609888

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KEYWORDS

Fluorescence lifetime imaging

In vivo imaging

Sensors

In vitro testing

Detector development

Fluorescence resonance energy transfer

Luminescence

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