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7 March 2022 Power efficient, uniform TIRF illumination via a photonic lantern for quantitative super-resolution imaging
Benjamin Croop, Juan Carlos Alvarado-Zacarias, Rodrigo Amezcua-Correa, Kyu Young Han
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Proceedings Volume PC11953, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXII; PC1195307 (2022) https://doi.org/10.1117/12.2608193
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Total internal reflection fluorescence (TIRF) is a popular type of illumination used in fluorescence microscopy for surface imaging of biospecimens; however, the commonly used objective-based TIRF illumination can induce artifacts that degrade the image. We previously demonstrated a fiber bundle that reshaped an input beam to achieve artifact-free TIRF illumination with coherent or incoherent light sources. Here we demonstrate an improved design using a photonic lantern, which will increase the power throughput by reducing coupling losses at the input facet. The improved power efficiency will facilitate new capabilities such as super-resolution imaging.
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Benjamin Croop, Juan Carlos Alvarado-Zacarias, Rodrigo Amezcua-Correa, and Kyu Young Han "Power efficient, uniform TIRF illumination via a photonic lantern for quantitative super-resolution imaging", Proc. SPIE PC11953, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXII, PC1195307 (7 March 2022); https://doi.org/10.1117/12.2608193
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KEYWORDS
Super resolution
Luminescence
Objectives
Microscopy
Reflection
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