Super-resolution algorithm for lensless microscope based on z-axis correction

Yang Chen; Linpeng Lu; Xuejuan Wu; Jialin Zhang; Chao Zuo

doi:10.1117/12.2601263

9 October 2021 Super-resolution algorithm for lensless microscope based on z-axis correction

Yang Chen, Linpeng Lu, Xuejuan Wu, Jialin Zhang, Chao Zuo

Proceedings Volume 11898, Holography, Diffractive Optics, and Applications XI; 118981F (2021) https://doi.org/10.1117/12.2601263
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Abstract

In traditional lensless in-line holographic microscopy, phase recovery based on multi-defocus distance is a common pixel-super-resolution technique. This method usually requires accurate displacement as a predictive condition, and the introduction of a precision mechanical displacement platform can bring about accurate displacement, but makes a simple lensless system complicated and expensive. In this paper, the samples are illuminated by a nearly coherent illumination system, and holograms at different heights are captured by the sensor driven by a low-cost servo motor without feedback system. Inaccurate displacement interval results in poor phase recovery. We propose a multi-height phase recovery algorithm based on z-axis correction to recover the phase information of the sample, which can improve the result by 1.58 times compared with that before correction. The reconstruction USAF target demonstrates a half-pitch lateral resolution of 775 nm across a large field-ofview of ∼29.84 mm² , surpassing 2.15 times that of the theoretical Nyquist–Shannon sampling resolution limit imposed by the pixel size of the imaging sensor （1.67 μm）.

Citation Download Citation

Yang Chen, Linpeng Lu, Xuejuan Wu, Jialin Zhang, and Chao Zuo "Super-resolution algorithm for lensless microscope based on z-axis correction", Proc. SPIE 11898, Holography, Diffractive Optics, and Applications XI, 118981F (9 October 2021); https://doi.org/10.1117/12.2601263

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