Assessment of murine gut dysbiosis with intraluminal fluorescence lifetime imaging

Alba Alfonso García; Stephanie A. Cevallos; Jee-Yon Lee; Cai Li; Julien Bec; Andreas Bäumler; Laura Marcu

doi:10.1117/12.2578687

5 March 2021 Assessment of murine gut dysbiosis with intraluminal fluorescence lifetime imaging

Alba Alfonso García, Stephanie A. Cevallos, Jee-Yon Lee, Cai Li, Julien Bec, Andreas Bäumler, Laura Marcu

Proceedings Volume 11626, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2021; 116260I (2021) https://doi.org/10.1117/12.2578687
Event: SPIE BiOS, 2021, Online Only

Abstract

Gastrointestinal disorders such as colorectal cancer or inflammatory bowel disease are linked to gut dysbiosis, an unbalanced gut microbiota. This early manifestation of the disease alters colon epithelial metabolism influencing the gut autofluorescence emission, which is susceptible to carry diagnostic value. We analyzed the fluorescence properties of healthy and dysbiotic ex vivo murine colons with an intraluminal fiber-based fluorescence lifetime imaging (FLIm) instrument. The results indicate that fluorescence lifetime reacts to inflammation in a spectrally dependant manner, and the full-length colon images allow to localize specific areas of activity. Imaging results were correlated to biochemical metabolic readouts (i.e. intracellular NADH, lactate) to establish the diagnostic potential of intraluminal FLIm.

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Alba Alfonso García, Stephanie A. Cevallos, Jee-Yon Lee, Cai Li, Julien Bec, Andreas Bäumler, and Laura Marcu "Assessment of murine gut dysbiosis with intraluminal fluorescence lifetime imaging", Proc. SPIE 11626, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2021, 116260I (5 March 2021); https://doi.org/10.1117/12.2578687

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KEYWORDS

Colon

Fluorescence lifetime imaging

Tissues

Mode conditioning cables

Luminescence

Switches

Diagnostics

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