Photoacoustic lifetime based oxygen imaging with G2 polyacrylamide nanosonophores for in vivo tumor models

Janggun Jo; Chang Lee; Raoul Kopelman; Xueding Wang

doi:10.1117/12.2577259

5 March 2021 Photoacoustic lifetime based oxygen imaging with G2 polyacrylamide nanosonophores for in vivo tumor models

Janggun Jo, Chang Lee, Raoul Kopelman, Xueding Wang

Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116422P (2021) https://doi.org/10.1117/12.2577259
Event: SPIE BiOS, 2021, Online Only

Abstract

We report photoacoustic (PA) lifetime (PALT) based oxygen imaging powered by polyacrylamide (PAA) nanoparticles (NPs). The phosphorescenc indicator, oxyphor G2, was conjugated within the matrix of the NPs, giving G2-PAA NPs, followed by PEGylation for biocompatibility and F3 surface modification for tumor targeting. Two laser pulses (630 nm pumping, 920 nm probing) excite the G2-PAA NPs. By varying the delay time between the pump and the probe pulses, the exponential decay of the phosphorescence, which correlates linearly with the ambient oxygen concentration, can be measured. PALT imaging was performed, to quantitatively map the oxygen concentration in biological tissues in vivo, including both cancerous tumors and normal thigh muscles. The successful imaging experiment accomplished on animal models renders a new method for in vivo non-invasive imaging and assessment of hypoxic tumor microenvironments, which is critical for assessing cancer progression, metastasis, and treatment.

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Janggun Jo, Chang Lee, Raoul Kopelman, and Xueding Wang "Photoacoustic lifetime based oxygen imaging with G2 polyacrylamide nanosonophores for in vivo tumor models", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422P (5 March 2021); https://doi.org/10.1117/12.2577259

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KEYWORDS

Tumors

Oxygen

In vivo imaging

Photoacoustic spectroscopy

Nanoparticles

Phosphorescence

Tissue optics

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