Towards easier, faster, super-resolved microscopy

Frederik Görlitz; Riccardo Wysoczanski; Sunil Kumar; Jonathan Lightley; Edwin Garcia; Yuriy Alexandrov; Ian Munro; Simon Johnson; Martin Kehoe; Callum Hollick; Jeremy Graham; Louise Donnelly; Peter Barnes; Christopher Dunsby; Mark A. A. Neil; Paul M. W. French

doi:10.1117/12.2550682

3 March 2020 Towards easier, faster, super-resolved microscopy

Frederik Görlitz, Riccardo Wysoczanski, Sunil Kumar, Jonathan Lightley, Edwin Garcia, Yuriy Alexandrov, Ian Munro, Simon Johnson, Martin Kehoe, Callum Hollick, Jeremy Graham, Louise Donnelly, Peter Barnes, Christopher Dunsby, Mark A. A. Neil, Paul M. W. French

Author Affiliations +

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460R (2020) https://doi.org/10.1117/12.2550682
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Super-resolved microscopy techniques have overcome the diffraction limit to provide image resolutions approaching the scale of fluorescent labels. However, many of these techniques require significant experimental resources and expertise and impose long image data acquisition times, making it difficult to acquire super-resolved data from sufficiently large sample numbers to overcome intrinsic biological variation. We have worked to make stimulated emission depletion (STED) microscopy and single molecule localisation microscopy (SMLM) more straightforward to implement and more practical to image larger numbers of cells. Here we present work in progress developing easySLM STED and easySTORM, including a new modular microscope frame that we believe can make it easier to prototype microscopy techniques and to implement and maintain them in lower resourced settings.

Citation Download Citation

Frederik Görlitz, Riccardo Wysoczanski, Sunil Kumar, Jonathan Lightley, Edwin Garcia, Yuriy Alexandrov, Ian Munro, Simon Johnson, Martin Kehoe, Callum Hollick, Jeremy Graham, Louise Donnelly, Peter Barnes, Christopher Dunsby, Mark A. A. Neil, and Paul M. W. French "Towards easier, faster, super-resolved microscopy", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460R (3 March 2020); https://doi.org/10.1117/12.2550682

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available