Time-resolved fluorescence microscopy for probing mitochondrial metabolites

Michael H. Gschwend; Wolfgang S. L. Strauss; Herbert Schneckenburger; Rudolf W. Steiner

doi:10.1117/12.229996

15 January 1996 Time-resolved fluorescence microscopy for probing mitochondrial metabolites

Michael H. Gschwend, Wolfgang S. L. Strauss, Herbert Schneckenburger, Rudolf W. Steiner

Proceedings Volume 2628, Optical and Imaging Techniques for Biomonitoring; (1996) https://doi.org/10.1117/12.229996
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

A novel set-up for time-gated (nanosecond) detection of fluorescence spectra and images of microscopic samples was recently developed. The apparatus was now used to measure the fluorescence of mitochondrial coenzymes (NADH, flavins) and a marker molecule (rhodamine 123) in endothelial cells from calf aorta. In these cultivated cells the electron transport chain was inhibited at various sites of the inner mitochondrial membrane. It could be shown that the fluorescence intensity of the free coenzyme NADH depended on the site of inhibition. In addition, an increased energy transfer from mitochondrial coenzymes (NADH, flavins) to rhodamine 123 molecules was observed, if the inhibition occurred in complex I (NADH- coenzyme Q reductase) or complex III (coenzyme QH₂-cytochrome c reductase) of the respiratory chain. The diagnostic potential of these findings are discussed.

Citation Download Citation

Michael H. Gschwend, Wolfgang S. L. Strauss, Herbert Schneckenburger, and Rudolf W. Steiner "Time-resolved fluorescence microscopy for probing mitochondrial metabolites", Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); https://doi.org/10.1117/12.229996

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