5-ALA photopreparation using pulsed NIR enhances skin fluorescence via temperature-independent cell signaling pathways

Augustin C. Barolet; Gregory Cormack; Daniel Barolet M.D.

doi:10.1117/12.2290507

8 February 2018 5-ALA photopreparation using pulsed NIR enhances skin fluorescence via temperature-independent cell signaling pathways

Augustin C. Barolet, Gregory Cormack, Daniel Barolet M.D.

Author Affiliations +

Proceedings Volume 10477, Mechanisms of Photobiomodulation Therapy XIII; 104770K (2018) https://doi.org/10.1117/12.2290507
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The effect of near infrared light (940 nm) on the conversion of 5-aminolevulinic acid (5-ALA) to PpIX, a compound involved in photodynamic therapy (PDT), was examined. The back skin of three test subjects was irradiated with continuous wavelength and pulsed infrared light at 940 nm. These irradiations took place 50-53, 24-29, and 8-14 hours prior to the application of the 5-ALA. After a three-hour incubation period with 5-ALA, a FluoDerm™device was used to measure the fluorescence of the skin (emitting wavelength: 400-420 nm; measuring excitation wavelength: 610-720 nm), a direct indication of the activity of 5-ALA. 5-ALA must penetrate the skin and then be converted to PpIX before any fluorescence increase can be observed. Results: For two patients (one was disqualified), the continuous wavelength, 50 hour pre-irradiation condition, the FluoDerm readings showed a 19 to 23% increase in fluorescence (p = 0.05) compared to the no-irradiation, 5-ALA only control.

Citation Download Citation

Augustin C. Barolet, Gregory Cormack, and Daniel Barolet M.D. "5-ALA photopreparation using pulsed NIR enhances skin fluorescence via temperature-independent cell signaling pathways", Proc. SPIE 10477, Mechanisms of Photobiomodulation Therapy XIII, 104770K (8 February 2018); https://doi.org/10.1117/12.2290507

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