MG63 cells behavior on rough polypyrrole scaffolds investigated by digital holographic microscopy

M. Mihailescu; A. Matei; A. Acasandrei; R. C. Popescu; I. A. Paun; Maria Dinescu

doi:10.1117/12.2062016

18 August 2014 MG63 cells behavior on rough polypyrrole scaffolds investigated by digital holographic microscopy

M. Mihailescu, A. Matei, A. Acasandrei, R. C. Popescu, I. A. Paun, Maria Dinescu

Proceedings Volume 9204, Interferometry XVII: Advanced Applications; 92040N (2014) https://doi.org/10.1117/12.2062016
Event: SPIE Optical Engineering + Applications, 2014, San Diego, California, United States

Abstract

The study of cells-substrate interaction became a stringent subject in the past decades, since an increasing variety of new materials and methods have been involved in tissue engineering or implants techniques. The investigation of this interaction using optical methods is a challenge, especially since these substrates are not optically polished. Due to their roughness in the micrometric or submicrometric range, the polymeric substrates offers good conditions for cells adhesion, but the characterization of cells properties can be hindered. In this study, we use Polypyrrole thin films, acting as substrates for cultured osteoblast-like MG63 cells having applications in tissue engineering for in vivo-like scaffolds. As characterization technique, we chose digital holographic microscopy, a single-shot technique, to obtain quantitative information about the sample features in a plane perpendicular to the substrate. Different parameters were tested in the experimental setup with the aim of finding the optimal conditions for details visualization. The reconstructed 3D images were filtered using a combination of analytical and implicit functions from MATLAB to exclude small/large objects, which correspond to Polypyrrole droplets to clearly identify the cells contour for quantitative measurements regarding their dimensions. These data were correlated with the effects on osteoblasts viability and differentiation. Also, the thickness and the refractive index of the substrate were determined using the decoupling procedure.

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M. Mihailescu, A. Matei, A. Acasandrei, R. C. Popescu, I. A. Paun, and Maria Dinescu "MG63 cells behavior on rough polypyrrole scaffolds investigated by digital holographic microscopy", Proc. SPIE 9204, Interferometry XVII: Advanced Applications, 92040N (18 August 2014); https://doi.org/10.1117/12.2062016

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KEYWORDS

Digital holography

Refractive index

Holograms

Polymers

Microscopy

Holography

3D image reconstruction

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