Candida albicansに対するamphotericin B及びflucytosineの併用効果に関する研究 Flow cytometryによる検討

田口 英昭; 田中 玲子; 宮治 誠; 西村 和子; 菅野 治重; 石山 尚子

doi:10.11150/kansenshogakuzasshi1970.66.516

Studies on the Effect of Combination of Amphotericin B and Flucytosine on Candida albicans by Flow Cytometry

Hideaki TAGUCHI, Reiko TANAKA, Makoto MIYAJI, Kazuko NISHIMURA, Harushige KANNO, Hisako ISHIYAMA

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Keywords: flow cytometry, amphotericin B, flucytosine, Candida albicans, synergism

JOURNAL FREE ACCESS

1992 Volume 66 Issue 4 Pages 516-521

DOI https://doi.org/10.11150/kansenshogakuzasshi1970.66.516

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Abstract

The antifungal effects of amphotericin B (AMPH) and flucytosine (5-FC) on three strains of Candida albicans were studied by flow cytometry (FCM).
When the IFM4954 and IFM4949 were treated with AMPH or 5-FC alone, cytograms of cell distribution changed at the concentration of 1/2 MIC, respectively.
Next, the effect of combination of AMPH and 5-FC were examined. Cytogram of cell distribution of IFM4954 and IFM4949 changed under the combination of 1/4 MIC of AMPH and 1/4 MIC of 5-FC, namely a synergic effect was observed.
In conclusion, FCM is a useful apparatus for analysis of the effect of antifungal agants.

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