It is well known that the broad spectrum antibiotic effect can cause abnormal growth of fungi such as candida in digestive tracts. In this consideration clinical experiments were attempted with combined preparation of Tetracycline (TC) and Amphotericin B (AMPH) known as an antifungal antibiotic.
Feces was cultured on Candida GS media and candida grown on it were counted. One tablet of this preparation contains 250 mg of TC and 50 mg of AMPH.
The results were as follows:
In twenty four healthy adults without any antibiotic given, average count of candida in feces was estimated to be 1.5×10³/g.
In nine in-patients with infectious diseases, mostly lung tuberculosis, daily dose of 16-18 tablets of TC-AMPH, four or three times per day, was continuously given orally and changing count of candida in feces was checked daily. In all cases tested, inhibitory effect to candida was evidently shown during the medication, even its disappearance being seen in some cases. The status reversed after its discontinuation.
The similar results were obtained in mice experiments.
The sensitivity of 37 strains of candida isolated from human feces in this institute against AMPH was determined with agar plate dilution method using Candida GS media. The MIC value turned out to be within 0.1-0.8mcg/ml.