Identification of bioactive compounds from onion (Allium burdickii ) bulb using Raman, and FTIR spectroscopy
Abstract
Purpose
This study aims to identify the bioactive compounds in the Onion (Allium burdickii [A.B]) bulb using Raman and Fourier transform infrared spectroscopy (FTIR) spectroscopy. It assessed the extraction conditions of bioactive compounds from A.B. while evaluating the best extraction conditions.
Design/methodology/approach
The research opted for an experimental qualitative approach. It examined the extraction conditions of A.B., namely, temperature (°C), time (min) and mass-to-liquor ratio (M:R) using ultraviolet-visible spectrophotometry. Identification of bioactive compounds present in the dye was performed using Raman spectroscopy and the validation of the results was done by FTIR spectroscopy.
Findings
The study determined the best extraction conditions (time, temperature, M:R) for A.B bulb. The study confirmed the presence of bioactive compounds.
Research limitations/implications
The limitation was quantification of bioactive compounds in A.B bulb.
Practical implications
The findings prove that the A.B. bulb can provide a sustainable source of bioactive compounds (functionalized compounds). The study provides suitable extraction conditions for A.B. and further elaborates on the techniques for identifying bioactive compounds in A.B. bulb extracts.
Social implications
The study provides A.B. as a source of bioactive compounds and a clean dye for textile coloration.
Originality/value
To the best of the authors’ knowledge, there is no documented study on the qualitative analysis of bioactive compounds in A.B using Raman and FTIR. Therefore, the study fulfils the identified need to ascertain alternative procedures for the analysis of bioactive compounds.
Keywords
Acknowledgements
The authors acknowledge financial assistance from African Centre of Excellence II in Phytochemicals, Textiles and Renewable Energy, Moi University (ACE II-PTRE), grant no ACE II-PTRE Project and Materials and Textile Laboratory, Busitema University for the facilities to do this work. The authors thank Nakyesse Eva, Mqondisi Moyo and Alsadiq Alamin Ibrahim for the advice on statistical software application.
The authors declare no existence of the conflict of interest.
Conceptualization, A.K.D., G.J.T., D.B., O.N.E., methodology, sample preparation, and formal analysis, A.K.D., writing original draft, A.K.D., writing-review and editing, G.J.T., D.B., visualization, A.K.D., D.B., supervision, G.J.T, O.N.E.
Citation
Agulei, K.D., Githaiga, J.T., Dulo, B. and Nganyi, E.O. (2023), "Identification of bioactive compounds from onion (
Publisher
:Emerald Publishing Limited
Copyright © 2023, Emerald Publishing Limited