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Volume 73, Issue 11

sp. nov. and sp. nov, two novel methylotrophic methanogens isolated from mangrove sediments in China No Access

Abstract

Two methylotrophic methanogens, designated strains FTZ2 and FTZ6, were isolated from mangrove sediment sampled in Futian Mangrove Nature Reserve in Shenzhen, PR China. Cells of strains FTZ2 and FTZ6 were cocci, with diameters of 0.6–1.0 µm and 0.6–0.9 µm, respectively. Both strains grew on methanol, methylamine, dimethylamine and trimethylamine, but not on acetate, formate, H/CO, choline, betaine or dimethyl sulphide. Strain FTZ2 grew at 10–37 °C (optimally at 33 °C), pH 5.5–8.0 (optimally at pH 7.0) and 0–1.03 M NaCl (optimally at 0.17 M NaCl). In contrast, strain FTZ6 grew at 15–42 °C (optimally at 37 °C), pH 5.0–7.5 (optimally pH 6.5) and 0–1.03 M NaCl (optimally at 0.17 M NaCl). Both strains required magnesium for growth and were susceptible to sodium dodecyl sulphate. Biotin was required for the growth of strain FTZ2 but not of strain FTZ6. The genomic G+C contents of strains FTZ2 and FTZ6 were 41.6 and 40.9 mol%, respectively. Phylogenetic analyses revealed that strain FTZ2 was mostly related to YSF-03, with 16S rRNA gene similarity of 98.6 %, an average nucleotide identity (ANI) of 82.5 %, and a digital DNA–DNA hybridization (dDDH) of 24.6 %. While strain FTZ6 was mostly related to PL-12/M, with 16S rRNA gene similarity of 99.4 %, an ANI of 88.6% and a dDDH of 34.6 %. Based on phenotypic, phylogenetic and genotypic evidence, two novel species of the genus , sp. nov. and sp. nov., are proposed. The type strain of sp. nov. is FTZ2 (=CCAM 1276=JCM 39396) and the type strain of sp. nov. is FTZ6 (=CCAM 1277=JCM 39397).

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  • Accepted:
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Keyword(s): archaea , mangrove sediments , Methanolobus and methylotrophic methanogen
Funding
This study was supported by the:
  • Shenzhen University 2035 Program for Excellent Research (Award 2022B002)
    • Principle Award Recipient: MengLI
  • Shenzhen Science and Technology Program (Award KCXFZ20201221173404012)
    • Principle Award Recipient: NotApplicable
  • Shenzhen Science and Technology Program (Award JCYJ20200109105010363)
    • Principle Award Recipient: MengLI
  • Innovation Team Project of Universities in Guangdong Province (Award 2020KCXTD023)
    • Principle Award Recipient: MengLI
  • China Postdoctoral Science Foundation (Award 2021TQ0212)
    • Principle Award Recipient: JinjieZhou
  • National Natural Science Foundation of China (Award 42007217)
    • Principle Award Recipient: Cui-JingZhang
  • National Natural Science Foundation of China (Award 32070108)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 32225003, 31970105, 92251306)
    • Principle Award Recipient: MengLI
  • National Natural Science Foundation of China (Award 42207144)
    • Principle Award Recipient: JinjieZhou
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100700)
    • Principle Award Recipient: MengLI
© 2023 The Authors
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Methanolobus mangrovi sp. nov. and Methanolobus sediminis sp. nov, two novel methylotrophic methanogens isolated from mangrove sediments in China
Int J Syst Evol Microbiol 73, 006169 (2023); https://doi.org/10.1099/ijsem.0.006169
/content/journal/ijsem/10.1099/ijsem.0.006169
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