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Volume 73, Issue 9

sp. nov., isolated from a eutrophic reservoir during the bloom period No Access

Abstract

A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, HBC54, was isolated from periphyton during a bloom. Based on the results of the 16S rRNA gene sequence analysis, strain HBC54 was closely related to 4Y4 (98.36 %), DSM 12444 (98.08 %), c7 (97.94 %), c1 (97.65 %), DSM 12447 (97.58 %), TW-4 (97.58 %) and UCT-28 (97.37 %). The average nucleotide identity and digital DNA–DNA hybridization values between HBC54 and its related type stains were below 78.97 and 23.7 %, which are lower than the threshold values for species delineation. The major fatty acids (>10.0 %) were identified as C 2-OH, summed feature 3 (C 7 and/or C 6) and summed feature 8 (C 7 and/or C 6) and the respiratory quinone was ubiquinone Q-10. The main polar lipids detected in the strain were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and three unidentified phospholipids. The genomic DNA G+C content was 64.8 mol%. Strain HBC54 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HBC54 (=KCTC 92033=LMG 32427).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): freshwater , genome sequencing , Novosphingobium cyanobacteriorum , periphyton and polyphasic taxonomy
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5252322)
    • Principle Award Recipient: Chi-YongAhn
  • National Research Foundation (Award 2023R1A2C1003308)
    • Principle Award Recipient: Chi-YongAhn
  • Korea Environmental Industry and Technology Institute (Award 2022003050004)
    • Principle Award Recipient: Chi-YongAhn
© 2023 The Authors
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2023-09-22
2024-04-28
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Novosphingobium cyanobacteriorum sp. nov., isolated from a eutrophic reservoir during the Microcystis bloom period
Int J Syst Evol Microbiol 73, 006042 (2023); https://doi.org/10.1099/ijsem.0.006042
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