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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

Description of gen. nov., sp. nov., sp. nov. and sp. nov., and genome-based taxonomy of genera and No Access

Abstract

Three extremely halophilic archaeal strains (LT55, SQT-29-1 and WLHS5) were isolated from Gobi saline soil and a salt lake, China. These strains were most related to the genera and (92.6–95.1 % similarities), and showed low similarities with other genera within the family based on 16S rRNA genes. Phylogenomic analysis confirmed that the three strains formed a distinct clade separated from the related genera and , which indicated that they may represent a novel genus of the family . The average nucleotide identity (ANI), in silico DNA–DNA hybridization (DDH) and average amino acid identity (AAI) values among the three strains were no more than 87, 34 and 85 %, respectively, much lower than the threshold values for species demarcation. The major phospholipids of the three strains were phosphatidic acid (PA), phosphatidylglycerol (PG) and phosphatidylglycerol phosphate methyl ester (PGP-Me). The glycolipid profiles of the three strains were diverse; sulfated mannosyl glucosyl diether (S-DGD-1) and disulfated mannosyl glucosyl diether (S-DGD) were found in strains LT55 and WLHS5, while mannosyl glucosyl diether (DGD-1) and S-DGD-1 in strain SQT-29-1. The combination of phenotypic, chemotaxonomic, phylogenetic and genomic analyses suggested that strains WLHS5 (=CGMCC 1.13781 = JCM 33558), SQT-29-1 (=CGMCC 1.16065 = JCM 33554) and LT55 (=CGMCC 1.15188 = JCM 30838) represent three novel species of a new genus within the family , for which the names, gen. nov., sp. nov., sp. nov. and sp. nov., are proposed. Genome-based classification of genera and revealed that should be transferred to the genus as comb. nov. and as a heterotypic synonym of Liu . 2015.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Halosolutus , Halovarius , Natrialbaceae , Natribaculum , saline soil and salt lake
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070003)
    • Principle Award Recipient: Heng-LinCui
© 2022 The Authors
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2022-10-26
2024-04-27
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Description of Halosolutus amylolyticus gen. nov., sp. nov., Halosolutus halophilus sp. nov. and Halosolutus gelatinilyticus sp. nov., and genome-based taxonomy of genera Natribaculum and Halovarius
Int J Syst Evol Microbiol 72, 005598 (2022); https://doi.org/10.1099/ijsem.0.005598
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